How to do nucleic acid testing for frozen food

We know that the rapid detection and sampling of human neocorona nucleic acid is mainly through nasopharyngeal swabs or oropharyngeal swabs. So how to do rapid detection of new crown nucleic acid for frozen food? In fact, the rapid detection of COVID-19 for food is similar to the rapid detection of human COVID-19. Next, let’s take a look at popular science for you:

1. Collection of seafood, fish and water samples

First open the gills of the fish sample, take a sampling swab, wipe 5-10 times on the gills, then put the sampling swab head into the collection tube containing the preservation solution, break the handle, and then seal it for inspection. ; Store the ice water sample of the fish, use a disposable plastic straw to suck 3-5ml into a 5ml preservation tube, and then seal it for inspection.

2. Frozen food for nucleic acid testing

For the rapid detection of COVID-19 on frozen foods, priority should be given to the collection of tissues with obvious diseased organs; secondly, aquatic products should be given priority to samples with internal organs and surrounding tissues; again, the same animal should be collected in the order of the sample types listed in the above table. The swab must be applied at the same time. No more than 3 samples can be collected from the same animal; finally, two samples are collected for each sample, one for testing and one for backup.

Animal-Genomic-DNA2

In addition to applying a swab on the surface of the food, the tissue also needs to be homogenized (homogenization is also called homogenization, which is a process of micronizing and homogenizing the dispersion in the suspension (or emulsion) system. This treatment simultaneously reduces the size of the dispersion and improves the uniformity of the dispersion), and then performs the normal rapid detection of new coronavirus nucleic acid, that is, reagent preparation, nucleic acid extraction, amplification detection, and issuance of reports.

3. Sampling of the surface of the object

Mainly cutting boards, knives, vegetables, meat, etc. First take a sampling swab, after soaking it in the preservation solution (if the surface of the object is wet, you can directly sample), wipe it horizontally and vertically on the surface of the object 5 times, and then put the sampling swab head into the collection tube containing the preservation solution. The handle is broken off, and then sealed and sent for inspection.

At present, most of the sporadic epidemics in many places are caused by cold chain foods, so the rapid detection of new crown nucleic acid in cold chain foods should also be attached great importance. It is necessary to strictly follow the sampling specifications and carefully sample the food and environment that may be contaminated. The rapid detection and sampling of new crown nucleic acids are mainly carried out for various types of frozen food, food packaging, related supplies and related staff, so as to achieve a comprehensive investigation, Leave no dead ends.