The kit is to configure the various raw materials that need to be used in PCR replication and amplification in advance, and then the medical staff can greatly save the configuration time when making the diagnosis. At the same time, the conditions (temperature, time, etc.) used by the kit also need to be explored and explored by the developer. Continuous optimization, testing and verification are required to ensure the accuracy and precision of the kit and avoid misdiagnosis and missed diagnosis. In the case of (false positive and false negative), a standardized novel coronavirus nucleic acid detection kit for clinical diagnosis can be obtained subsequently.
1. What is the rapid detection of new coronavirus nucleic acid?
As we all know, rapid nucleic acid testing is currently the “gold standard” for the detection of new coronaviruses, and it is also a direct evidence for judging whether there is a new coronavirus in the subject. The new coronavirus is an RNA virus, and the specific RNA sequence in the virus is a marker that distinguishes the virus from other pathogens. After the emergence of the new type of coronavirus, Chinese scientists completed the analysis of the complete genome sequence of the new type of coronavirus in a very short time, and discovered the specific nucleic acid sequence in the new type of coronavirus. If the specific nucleic acid sequence of the new coronavirus is detected in the patient’s sample, it indicates that the patient may be infected by the new coronavirus.
2. Principles of COVID-19 rapid detection kit
Most of the rapid nucleic acid detection kits use the fluorescent quantitative PCR method. The genetic material of organisms is divided into two types, DNA and RNA. DNA has a double helix-like structure, which is more stable, while RNA is a single-stranded structure. Coronavirus is an RNA virus, which is easier to mutate and adapt to the human body. The most important way to confirm whether a patient is infected with the new coronavirus is to detect whether the genetic material of the virus is present in the patient or suspected case.
However, the amount of RNA that can be extracted in patient samples is limited, so a method is needed to quickly detect the nucleic acid content in virus samples. Fluorescence quantitative PCR is a new type of quantitative test technology launched by an American company in 1996. It uses fluorescent dyes or fluorescently-labeled specific probes to label and track PCR products for real-time monitoring of the reaction. So, what is PCR? PCR is the polymerase chain reaction, which can greatly increase the trace amount of DNA. The process of fluorescent quantitative PCR is to use enzymes to generate DNA from RNA through reverse transcription reaction, and then use DNA as a template for amplification. At this time, more detectable fragments will be obtained.
At present, the rapid nucleic acid detection kits that use the fluorescent quantitative PCR method in China mainly have the advantages of high early diagnosis sensitivity, strong specificity, fast detection speed, and high popularity. For example, the new coronavirus nucleic acid rapid detection kit launched by Aisen Biotechnology Company, this new coronavirus nucleic acid rapid detection kit has obvious advantages of convenience, high sensitivity, strong specificity, and fast detection time, which greatly improves the new coronavirus Inspection efficiency can be widely used in clinical, CDC, and customs inspection and quarantine.