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African Swine Fever Virus Extraction
African Swine fever (ASF) is an infectious disease caused by African Swine fever virus (ASFV, double-stranded DNA virus, 170-190kb) infecting pigs. It is a type of animal epidemic that our country focuses on preventing , The mortality rate is nearly 100%.
There is currently no vaccine available for ASF prevention. Although ASF does not infect humans, it is a devastating blow to the pig industry. The mortality rate of pigs up to 100%. China is a big pig-raising country, and the Chinese people consume extremely large amounts of pork, amounting to 1.6 trillion yuan, driving other industries more than 3 trillion yuan.
The control problem has a great impact on the pig industry and even the national economy and people’s livelihood.
PCR is currently the most commonly used method for ASFV detection in laboratories and plays an important role in the early detection of ASFV. PCR has been successfully applied to the detection of ASFV genome in pig samples (blood, organs, etc.) and ticks. Viral DNA fragments are amplified by PCR to obtain a sufficient amount for detection, and PCR can quickly become ASF detection within a few hours. Compared with ELISA and direct fluorescent antibody test (FAT), PCR has higher sensitivity and specificity, can detect very low levels of ASFV, and can quickly detect viral nucleic acid in the early stage of pig infection with ASFV.